5CW5

Structure of CfBRCC36-CfKIAA0157 complex (QSQ mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28270
Spacegroup name	P 21 21 21
Unit cell lengths	89.452, 124.173, 132.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.939 - 2.736
R-factor	0.2329
Rwork	0.231
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.493
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.736	5.920	2.750
Rmerge	0.136	0.065	0.927
Rmeas	0.158	0.080
Rpim	0.094	0.045	0.664
Total number of observations	103925
Number of reflections	37786
<I/σ(I)>	4.2
Completeness [%]	95.8	93.3	93.2
Redundancy	2.8	2.7	2.6
CC(1/2)		0.992	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M KCl, 0.1 M HEPES-Na (pH 7.5) and 15% PEG6000