5CW3

Structure of CfBRCC36-CfKIAA0157 complex (Zn Edge)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.28240
Spacegroup name	P 21 21 21
Unit cell lengths	49.930, 116.580, 226.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.755 - 2.550
R-factor	0.2125
Rwork	0.211
R-free	0.25830
RMSD bond length	0.002
RMSD bond angle	0.504
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.755	7.500	2.650
High resolution limit [Å]	2.550	5.300	2.550
Rmerge	0.107	0.073	1.276
Rmeas	0.123	0.084	1.494
Total number of observations	277108
Number of reflections	80922	5696	8974
<I/σ(I)>	7.22	14.62	1.01
Completeness [%]	97.3	95.4	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Na HEPES (pH 7.0), 10% w/v PEG4000 and 10% (v/v) 2-propanol