5CW0

Investigation of RNA structure in satellite panicum mosaic virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2001-11-28
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	H 3
Unit cell lengths	175.470, 175.470, 418.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 4.600
R-factor	0.2818
Rwork	0.282
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1stm
RMSD bond length	0.014
RMSD bond angle	1.791
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.760
High resolution limit [Å]	4.600	4.600
Number of reflections	25998
<I/σ(I)>	5.788	1.98
Completeness [%]	97.4	95.4
Redundancy	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	6%-8% PEG 3350, pH 8.0-9.0