5CVZ

Investigation of RNA structure in satellite panicum mosaic virus - glutaraldehyde treated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 3
Unit cell lengths	226.350, 226.350, 226.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.23
Rwork	0.230
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1stm
RMSD bond length	0.009
RMSD bond angle	1.549
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	58624
<I/σ(I)>	27.725	6.48
Completeness [%]	100.0	100
Redundancy	17.2	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	6%-14% PEG 3350, pH 5.0-7.0. Gluteraldehyde-treated before freezing