5CVU
sinpyl alcohol bound monolignol 4-O-methyltransferase 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.540, 151.630, 68.390 |
| Unit cell angles | 90.00, 92.55, 90.00 |
Refinement procedure
| Resolution | 75.810 - 1.800 |
| R-factor | 0.1683 |
| Rwork | 0.168 |
| R-free | 0.16804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.360 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.810 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 156919 |
| <I/σ(I)> | 13.7 |
| Completeness [%] | 88.9 |
| Redundancy | 5.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | MOMT5 was grown via a hanging-drop vapor diffusion method in 22% (w/v) PEG 4000, 0.3 M Mg(NO3)2 (pH 7.2), and 1 mM DTT with 1 mM SAH and 1 mM sinapyl alcohol |
