5CVI

Structure of the manganese regulator SloR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-22
Detector	NOIR-1
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	60.432, 60.432, 298.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.230 - 2.804
R-factor	0.2025
Rwork	0.200
R-free	0.25840
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.230	2.960
High resolution limit [Å]	2.800	2.800
Rmerge	0.098	0.532
Number of reflections	25853
<I/σ(I)>	25	5.1
Completeness [%]	99.7	98.9
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	12% PEG 3350, 40 mM lithium sulfate, 1 mM zinc chloride