5CVI
Structure of the manganese regulator SloR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-22 |
Detector | NOIR-1 |
Wavelength(s) | 0.9785 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.432, 60.432, 298.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.230 - 2.804 |
R-factor | 0.2025 |
Rwork | 0.200 |
R-free | 0.25840 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.565 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.230 | 2.960 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.098 | 0.532 |
Number of reflections | 25853 | |
<I/σ(I)> | 25 | 5.1 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12% PEG 3350, 40 mM lithium sulfate, 1 mM zinc chloride |