5CVH
Crystal Structure of CK2alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9188 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.495, 68.690, 333.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 166.550 - 1.848 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3war |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 166.550 | 1.855 |
| High resolution limit [Å] | 1.848 | 1.848 |
| Rmerge | 0.040 | 0.852 |
| Number of reflections | 64008 | |
| <I/σ(I)> | 20.6 | 2.2 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 107mM Mes pH 6.5, 29% glycerol ethoxylate, 1 M ammonium acetate |
