5CV7
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-12-11 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.114, 60.293, 38.737 |
Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
Resolution | 38.640 - 1.650 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.843 |
Data reduction software | APEX |
Data scaling software | APEX |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.025 | 0.239 |
Total number of observations | 165896 | |
Number of reflections | 17257 | |
<I/σ(I)> | 26.6 | 3.57 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.33 | 3.63 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |