5CV3
C. remanei PGL-1 Dimerization Domain - Hg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 87.312, 87.312, 45.059 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.708 - 3.170 |
| R-factor | 0.223656523372 |
| Rwork | 0.219 |
| R-free | 0.26662 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.710 | 50.000 | 2.800 |
| High resolution limit [Å] | 3.170 | 7.320 | 2.750 |
| Rmerge | 0.126 | 0.083 | 0.557 |
| Number of reflections | 3523 | ||
| <I/σ(I)> | 28.62 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 7 | 12.9 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM PIPES pH 6.0, 24-27% PEG 4K, 200 mM LiSO4 |
