5CUZ
Crystal structure of SeMet-substituted N-terminal truncated human B12-chaperone CblD (108-296)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979475 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.341, 66.226, 71.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.710 - 2.310 |
| R-factor | 0.21101 |
| Rwork | 0.208 |
| R-free | 0.26266 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.098 | 0.359 |
| Number of reflections | 6667 | |
| <I/σ(I)> | 14.5 | 2.5 |
| Completeness [%] | 94.1 | 70.4 |
| Redundancy | 7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % PEG3350, 0.1M Tris-HCl, pH 7.5, 0.185 M MgCl2, 0.12 M NaF |
