5CUZ
Crystal structure of SeMet-substituted N-terminal truncated human B12-chaperone CblD (108-296)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979475 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.341, 66.226, 71.885 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.710 - 2.310 |
R-factor | 0.21101 |
Rwork | 0.208 |
R-free | 0.26266 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.374 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.098 | 0.359 |
Number of reflections | 6667 | |
<I/σ(I)> | 14.5 | 2.5 |
Completeness [%] | 94.1 | 70.4 |
Redundancy | 7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % PEG3350, 0.1M Tris-HCl, pH 7.5, 0.185 M MgCl2, 0.12 M NaF |