5CUP
Structure of Rhodopseudomonas palustris PduL - phosphate bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.098, 58.763, 136.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.227 - 2.100 |
R-factor | 0.1977 |
Rwork | 0.195 |
R-free | 0.22840 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.713 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.230 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.856 | |
Number of reflections | 27556 | |
<I/σ(I)> | 13.78 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.8 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | 35% MPD |