5CUL
crystal structure of the PscU C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-22 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 64 |
Unit cell lengths | 68.570, 68.570, 61.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.660 - 2.900 |
R-factor | 0.19701 |
Rwork | 0.196 |
R-free | 0.22452 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jli |
RMSD bond length | 0.012 |
RMSD bond angle | 1.110 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.660 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.387 | 1.799 |
Number of reflections | 3694 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 11 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1M di-ammonium hydrogen phosphate, 0.1 M sodium acetate, pH 4.5 |