5CUK
Crystal structure of the PscP SS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.140, 42.480, 25.860 |
| Unit cell angles | 90.00, 99.07, 90.00 |
Refinement procedure
| Resolution | 39.207 - 2.100 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.693 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.207 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.576 |
| Number of reflections | 6182 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 94.6 | 93.4 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M citric acid, 20% PEG 60000, pH 5.0 |
