5CU9

CANDIDA ALBICANS SUPEROXIDE DISMUTASE 5 (SOD5), APO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	34.531, 37.699, 102.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.438 - 1.480
R-factor	0.2071
Rwork	0.202
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n3t
RMSD bond length	0.005
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.870	1.560
High resolution limit [Å]	1.480	1.480
Rmerge		0.634
Number of reflections	22964
<I/σ(I)>	16.6	2.3
Completeness [%]	99.2	99.9
Redundancy	4.9	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	2.4 M ammonium sulfate, 0.1 M HEPES