5CTR
Crystal structure of human SART3 HAT-C domain-human USP4 DUSP-UBL domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 115.202, 115.202, 306.138 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.012 |
R-factor | 0.31 |
Rwork | 0.309 |
R-free | 0.33370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ctq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.305 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.107 | 0.413 |
Number of reflections | 41894 | |
<I/σ(I)> | 11.7 | 2.26 |
Completeness [%] | 98.0 | 96.2 |
Redundancy | 4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES pH 6.8, 200 mM magnesium chloride, 15% PEG 4000 |