5CTR

Crystal structure of human SART3 HAT-C domain-human USP4 DUSP-UBL domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	115.202, 115.202, 306.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.012
R-factor	0.31
Rwork	0.309
R-free	0.33370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ctq
RMSD bond length	0.006
RMSD bond angle	1.305
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.107	0.413
Number of reflections	41894
<I/σ(I)>	11.7	2.26
Completeness [%]	98.0	96.2
Redundancy	4	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES pH 6.8, 200 mM magnesium chloride, 15% PEG 4000