5CTG
The 3.1 A resolution structure of a eukaryotic SWEET transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0717 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.830, 142.240, 81.570 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 29.896 - 3.103 |
| R-factor | 0.2547 |
| Rwork | 0.253 |
| R-free | 0.29130 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.354 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.092 | 0.654 |
| Number of reflections | 21149 | |
| <I/σ(I)> | 16.2 | 1.6 |
| Completeness [%] | 98.9 | 96.2 |
| Redundancy | 6.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES, pH 6.5, 30% PEG400, 10mM MnCl2, 5% ethanol |
