5CST
Structure of the complex of type 1 ribosome inactivating protein from Momordica balsamina with a nucleotide, cytidine diphosphate at 1.78 A resolution
Replaces: 4ZW4Replaces: 4K6PReplaces: 4QGJExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.216, 130.216, 40.458 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.110 - 1.780 |
| R-factor | 0.15545 |
| Rwork | 0.151 |
| R-free | 0.18878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s9q |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.110 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 22958 | |
| <I/σ(I)> | 34 | 2.6 |
| Completeness [%] | 99.8 | 96.9 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate |
