5CSJ

S100B-RSK1 crystal structure B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	37.960, 68.930, 91.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.115 - 2.700
R-factor	0.2067
Rwork	0.203
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3czt
RMSD bond length	0.022
RMSD bond angle	1.376
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1750)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.120	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	7001
<I/σ(I)>	9.19	2.19
Completeness [%]	99.9	100
Redundancy	12.3	13.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M Hepes 7, 150 mM NaCl, 20% PEG6000