5CSI

S100B-RSK1 crystal structure A'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9730
Spacegroup name	P 21 21 21
Unit cell lengths	38.310, 38.570, 173.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.650 - 2.130
R-factor	0.2069
Rwork	0.205
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3czt
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1750)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.650	2.190
High resolution limit [Å]	2.130	2.130
Number of reflections	15160
<I/σ(I)>	18.77	2.07
Completeness [%]	99.9	100
Redundancy	6.43	6.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M Hepes 7, 150 mM NaCl, 20% PEG6000