5CSG
The crystal structure of beta2-microglobulin R97Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 57.353, 29.070, 61.240 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 38.820 - 1.500 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.580 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | |
| Number of reflections | 15172 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 93.5 | 91 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 27% PEG 4000, 0.1 M sodium acetate, 15% glycerol, 0.2 M ammonium acetate |
