5CS7
The crystal structure of wt beta2-microglobulin at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-05-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 59.630, 29.240, 65.956 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 39.006 - 2.100 |
| R-factor | 0.1801 |
| Rwork | 0.174 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.010 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | |
| Number of reflections | 6599 | |
| <I/σ(I)> | 7.6 | 2.3 |
| Completeness [%] | 99.1 | 97.9 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 30% PEG 4000, 0.1M sodium acetate, 15% glycerol, 0.2M ammonium acetate |
