5CS5
The structure of the NK1 fragment of HGF/SF complexed with PIPES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.775, 63.630, 57.395 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 26.060 - 1.900 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nk1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.146 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.044 | 0.259 |
Number of reflections | 30379 | |
<I/σ(I)> | 18.6 | |
Completeness [%] | 99.3 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19% PEG 4000, 200 mM Na Acetate, 150 mM Tris |