5CS3
The structure of the NK1 fragment of HGF/SF complexed with (H)EPPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-09 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.340, 63.378, 57.482 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 34.300 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.213 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nk1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.828 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.127 | 0.528 |
| Number of reflections | 13345 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 100.0 | 99.99 |
| Redundancy | 7.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19% PEG 4000, 200 mM Na Acetate, 150 mM Tris |
