5CS0
Crystal structure of domains AC1-AC2 of yeast acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 65 |
| Unit cell lengths | 117.619, 117.619, 73.856 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.140 - 2.500 |
| R-factor | 0.2151 |
| Rwork | 0.212 |
| R-free | 0.27090 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.071 | 0.048 | 0.416 |
| Total number of observations | 84727 | ||
| Number of reflections | 20265 | ||
| <I/σ(I)> | 13.8 | 2.6 | |
| Completeness [%] | 99.7 | 98.9 | 99.4 |
| Redundancy | 4.2 | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 90 mM Bis-tris propane and 60 mM citric acid, and 20% (w/v) PEG3350 |
