5CR9
Crystal structure of ABC-type Fe3+-hydroxamate transport system from Saccharomonospora viridis DSM 43017
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.690, 56.855, 62.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.569 - 1.700 |
| R-factor | 0.1471 |
| Rwork | 0.146 |
| R-free | 0.17220 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_1888) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.570 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.098 | 0.669 |
| Number of reflections | 33513 | |
| <I/σ(I)> | 29.76 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 273 | 40 % MPD, 0.1 M Tris pH 8.00 |
