5CR4

Crystal structure of the Sleeping Beauty transposase catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	I 21 21 21
Unit cell lengths	84.240, 113.980, 144.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.400
R-factor	0.1724
Rwork	0.171
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hos
RMSD bond length	0.020
RMSD bond angle	1.907
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.400	1.400
Number of reflections	136613
<I/σ(I)>	17.9	1.99
Completeness [%]	100.0	100
Redundancy	11.2	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	0.1 M sodium citrate, 3.2 M ammonium sulphate