5CR3
Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-06-20 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.065, 60.360, 38.526 |
Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
Resolution | 38.440 - 1.800 |
R-factor | 0.1808 |
Rwork | 0.178 |
R-free | 0.23240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.760 |
Data reduction software | APEX (2013/1 for Windows) |
Data scaling software | APEX (2013/1 for Windows) |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.046 | 0.216 |
Total number of observations | 83300 | |
Number of reflections | 13254 | |
<I/σ(I)> | 16.59 | 4.54 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.91 | 3.64 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |