5CR0

Human DNA polymerase lambda L431A mutant- MgdCTP binary and complex with 6 paired DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	95
Detector technology	CCD
Collection date	2013-06-29
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	84.511, 84.511, 399.101
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.507 - 2.750
R-factor	0.2467
Rwork	0.243
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.850
High resolution limit [Å]	2.750	5.910	2.750
Rmerge	0.057	0.027	0.746
Total number of observations	264376
Number of reflections	23254
<I/σ(I)>	17.1
Completeness [%]	99.9	99.2	100
Redundancy	11.4	12	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate