5CQY
E. coli MazF mutant E24A in complex with MazE residues 68-82 form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980100 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.170, 52.170, 197.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.044 - 2.481 |
| R-factor | 0.2003 |
| Rwork | 0.198 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.040 | 2.630 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.800 | |
| Number of reflections | 10603 | |
| <I/σ(I)> | 10.83 | 1.64 |
| Completeness [%] | 98.7 | 98.4 |
| Redundancy | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 24% w/v PEG1500, 20% v/v Glycerol |
