5CQV
Crystal structure of uncharacterized protein Q8DWV2 from Streptococcus agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-03 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91730 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.940, 65.930, 55.750 |
Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
Resolution | 20.160 - 1.900 |
R-factor | 0.1801 |
Rwork | 0.178 |
R-free | 0.21870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5com |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | xia2 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.160 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.040 | 0.439 |
Number of reflections | 26530 | |
<I/σ(I)> | 17.1 | 2.3 |
Completeness [%] | 98.2 | 97.6 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.12 M Diethylene glycol, 0.12 M Triethylene glycol, 0.12 M Tetraethylene glycol, 0.12 M Pentaethylene glycol, 0.1 M Tris, 0.1 M Bicine, pH 8.5, 5% MPD, 12% PEG 550 MME, 6% PEG 20,000 |