5CQI
Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.020, 54.290, 65.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.800 - 1.680 |
R-factor | 0.1623 |
Rwork | 0.160 |
R-free | 0.19780 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.815 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2166: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.800 |
High resolution limit [Å] | 1.680 |
Number of reflections | 16702 |
<I/σ(I)> | 17.9 |
Completeness [%] | 95.0 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350, MES-NaOH |