5CQH

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	50.843, 52.448, 68.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.700 - 1.730
R-factor	0.1541
Rwork	0.152
R-free	0.18590
RMSD bond length	0.018
RMSD bond angle	1.652
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((dev_2067: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.700
High resolution limit [Å]	1.730
Number of reflections	19558
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, MES-NaOH