5CQC

Crystal structure of the legionella pneumophila effector protein RavZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	I 4 2 2
Unit cell lengths	221.778, 221.778, 72.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.942 - 2.985
R-factor	0.218
Rwork	0.214
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.329
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.000	3.140
High resolution limit [Å]	2.980	2.980
Rmerge	0.066
Number of reflections	18194
<I/σ(I)>	17.3	0.9
Completeness [%]	99.2	98.2
Redundancy	5.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	277.15	12% PEG 3350, 0.2 M BaCl2, 0.1 M MES