5CPY
Crystal structure of murine polyomavirus PTA strain VP1 in complex with the GD1a glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 220.450, 220.450, 99.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.770 - 1.930 |
| R-factor | 0.1537 |
| Rwork | 0.153 |
| R-free | 0.17430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 199442 | |
| <I/σ(I)> | 12.8 | 3 |
| Completeness [%] | 96.0 | 98.1 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M HEPES, pH 7.5, 1 M sodium phosphate, monobasic, 0.8 M potassium phosphate, dibasic |
