5CPX
Crystal structure of murine polyomavirus PTA strain VP1 in complex with the DSLNT glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 219.730, 219.730, 100.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.360 - 1.870 |
| R-factor | 0.1544 |
| Rwork | 0.154 |
| R-free | 0.17560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 226957 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 0.1 M HEPES pH 8.5, 1 M sodium phosphate monobasic, 0.8 M potassium phosphate dibasic |
