5CPD

Methionine-alanine complex structure of peptide deformylase from Xanthomonas oryzae pv. oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.99
Spacegroup name	P 61 2 2
Unit cell lengths	58.584, 58.584, 266.188
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.400 - 2.200
R-factor	0.19393
Rwork	0.191
R-free	0.24724
RMSD bond length	0.021
RMSD bond angle	2.086
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	16778
<I/σ(I)>	9.8
Completeness [%]	98.5
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	0.05M cadmium sulfate, 0.1M HEPES pH 7.5, 2.0M sodium acetate trihydrate