5CP4

CRYOGENIC STRUCTURE OF P450CAM

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1996-04
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 21 21 21
Unit cell lengths	106.330, 103.300, 36.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.750
R-factor	0.21
Rwork	0.210
R-free	0.26000
Structure solution method	MR
Starting model (for MR)	2cpp
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.750	1.750
Rmerge	0.058 *
Total number of observations	179587 *
Number of reflections	37112 *
Completeness [%]	89.7	54.7
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7		Poulos, T.L., (1996) Methods Enzymol., 272, 358. *