5CP2

Human DNA polymerase lambda L431A mutant- Apoenzyme and complex with 6 paired DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	95
Detector technology	CCD
Collection date	2013-06-30
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	83.876, 83.876, 396.363
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.950 - 2.360
R-factor	0.209
Rwork	0.206
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.005
RMSD bond angle	0.823
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.430
High resolution limit [Å]	2.360	5.390	2.360
Rmerge	0.066	0.026	0.529
Number of reflections	35440
<I/σ(I)>	13
Completeness [%]	99.8	99.3	98.6
Redundancy	13.7	12.2	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate