5COZ
Crystal structure of an uncharacterized protein (EUBREC_2869) from Eubacterium rectale ATCC 33656 at 1.45 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.95369,0.97934,0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.090, 60.060, 130.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.263 - 1.450 |
| R-factor | 0.1617 |
| Rwork | 0.161 |
| R-free | 0.18540 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.263 | 29.263 | 1.490 |
| High resolution limit [Å] | 1.450 | 6.480 | 1.450 |
| Rmerge | 0.043 | 0.586 | |
| Rmeas | 0.099 | 0.050 | 0.687 |
| Rpim | 0.048 | 0.025 | 0.350 |
| Total number of observations | 267287 | 3069 | 16918 |
| Number of reflections | 66473 | ||
| <I/σ(I)> | 9.7 | 21.7 | 2 |
| Completeness [%] | 99.9 | 98.4 | 98.9 |
| Redundancy | 4 | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M sodium cacodylate pH 6.5, 30% polyethylene glycol 8000, 0.2M sodium acetate |
