5COY
Crystal structure of CC chemokine 5 (CCL5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.021 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.601, 56.196, 94.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.091 - 1.443 |
| R-factor | 0.1581 |
| Rwork | 0.155 |
| R-free | 0.19240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b3a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.337 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.046 | 0.414 |
| Number of reflections | 23175 | |
| <I/σ(I)> | 26 | 2.6 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Potassium phosphate monobasic, 20% w/v Polyethylene glycol 3,350 |
