5COT
Structure and mechanism of a eukaryal nick-sealing RNA ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-24 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 |
Unit cell lengths | 55.285, 55.285, 102.545 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.610 - 1.690 |
R-factor | 0.16046 |
Rwork | 0.158 |
R-free | 0.19953 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 2.183 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.051 | 0.414 |
Number of reflections | 38947 | |
<I/σ(I)> | 31.9 | 2.51 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 4.9 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES, 30% PEG 6000 |