5COS
Crystal Structure of the Cytoplasmic Domain of the Pseudomonas putida Anti-sigma Factor PupR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.459, 134.621, 34.896 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 33.655 - 2.019 |
| R-factor | 0.2177 |
| Rwork | 0.215 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cam |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 2.130 |
| High resolution limit [Å] | 2.019 | 2.020 |
| Number of reflections | 18320 | |
| <I/σ(I)> | 13.3 | 2.4 |
| Completeness [%] | 98.8 | 99.4 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100mM Bis-Tris pH 6.0, 3M NaCl |
