5COL
RIBOSOMAL PROTEIN L11 FROM METHANOCOCCUS JANNASCHII
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625, 0.97917 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 69.150, 88.050, 142.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.030 - 2.250 |
| R-factor | 0.21703 |
| Rwork | 0.215 |
| R-free | 0.25237 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.741 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.055 |
| Number of reflections | 22254 |
| <I/σ(I)> | 13.57 |
| Completeness [%] | 97.8 |
| Redundancy | 4.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 100 mM Sodium Citrate, pH 5.0, 0.1 M MgCl2, 27% PEG 600 |
