Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 244.832, 251.414, 412.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.687 - 6.740 |
| R-factor | 0.425205947788 |
| Rwork | 0.425 |
| R-free | 0.43465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uq8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.177 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 7.200 |
| High resolution limit [Å] | 6.740 | 6.800 |
| Rmerge | 0.083 | 1.270 |
| Number of reflections | 44280 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | Tris-HCl, magnesium chloride, sodium chloride, PEG 3350, n-Dodecyl beta-D-maltoside |
