5COC
Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-06-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | I 41 |
Unit cell lengths | 98.807, 98.807, 31.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.669 | 5.810 | 2.669 |
Rmerge | 0.099 | 0.043 | 0.576 |
Total number of observations | 26545 | ||
Number of reflections | 4463 | ||
<I/σ(I)> | 10.4 | ||
Completeness [%] | 99.6 | 97.9 | 99.5 |
Redundancy | 5.9 | 5.9 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 10% w/v PEG 1000, 10% w/v PEG 8000 |