5CO9
Crystal structure of human zinc insulin at pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-20 |
Detector | OXFORD TITAN CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 81.560, 81.560, 33.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.940 - 1.920 |
R-factor | 0.19055 |
Rwork | 0.188 |
R-free | 0.23734 |
Structure solution method | SIRAS |
RMSD bond length | 0.018 |
RMSD bond angle | 1.937 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 16.940 |
High resolution limit [Å] | 1.920 |
Number of reflections | 5999 |
<I/σ(I)> | 8.2 |
Completeness [%] | 97.6 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2 mcL protein (6 mg/mL) + 2 mcL well (0.1 M MES buffer pH 6.5, 1.6 M MgSO4, directly from the commercially available kit Hampton Crystal Screen II, formulation #20) |