5CO8
Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9750 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.072, 98.072, 119.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.036 - 2.400 |
| R-factor | 0.2396 |
| Rwork | 0.239 |
| R-free | 0.25470 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.877 |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.790 | 2.570 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.140 | 1.376 |
| Rmeas | 0.182 | |
| Rpim | 0.075 | 1.495 |
| Total number of observations | 218998 | 26404 |
| Number of reflections | 26454 | |
| <I/σ(I)> | 7.5 | 0.4 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 10.6 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 279 | PEG10000 NaCl magnesium chloride |
