5CO7
E. coli MazF form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 129.050, 129.050, 299.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.020 - 3.489 |
| R-factor | 0.2783 |
| Rwork | 0.276 |
| R-free | 0.32140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.020 | 3.700 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 1.220 | |
| Number of reflections | 32860 | |
| <I/σ(I)> | 12.85 | 1.8 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 14.6 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1M Imidazole pH 6.5, 1M Sodium Acetate |
