5CO5

Crystal structure of Ac-AChBP in complex with conotoxin GIC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-17
Detector	BRUKER SMART 6500
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	78.585, 84.941, 208.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.453 - 2.100
R-factor	0.1806
Rwork	0.178
R-free	0.22100
RMSD bond length	0.008
RMSD bond angle	1.153
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	85409
<I/σ(I)>	18.7
Completeness [%]	96.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	1.5M lithium sulfate monohydrate, 0.1M Tris pH 8.5