5CO1
Crystal Structure of Zebrafish Protocadherin-19 EC3-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.355, 86.631, 132.583 |
| Unit cell angles | 90.00, 122.13, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| R-factor | 0.19091 |
| Rwork | 0.188 |
| R-free | 0.23943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aqe_A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.392 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.591 | |
| Number of reflections | 47847 | |
| <I/σ(I)> | 16.59 | 2.21 |
| Completeness [%] | 98.0 | 88.1 |
| Redundancy | 4.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 277 | 100 mM Sodium Cacodylate pH 6.1, 100 mM Calcium Acetate, 25% MPD |
